rat anti ed 1 cd68  (Bio-Rad)

Journal: Communications Biology

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network

doi: 10.1038/s42003-022-03712-2

Figure Lengend Snippet: a Experimental scheme depicting the induction of anti-GBM nephritis followed by intravenous ASC and BMMSC administration on days 0, 2, and 4. b Serum creatinine level on days 3 and 7 (day 3, n = 5 per group; day 7, n = 8 per group). c Urinary protein excretion on day 3 ( n = 8 per group). d Representative images of kidney sections stained with Periodic Acid-Schiff on day 7 (left) and glomerular injury scoring based on crescent formation on days 3 and 7 (right). e Representative images of kidney sections stained with anti-CD68 Ab on day 7 (left) and the number of CD68-positive cells in the glomerulus on days 3 and 7 (right). Scale bars, 50 μm. (day 3, n = 5 per group; day 7, n = 8–9 per group). All data are shown as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, as determined by ANOVA. ASC adipose-derived MSC, BMMSC bone marrow-derived MSC.

Article Snippet: To evaluate macrophage infiltration, formalin-fixed kidneys were stained using mouse anti-rat CD68 monoclonal IgG1 (clone ED-1; BMA Biomedicals, Augst, Switzerland) as a marker for infiltrating macrophages or dendritic cells.

Techniques: Staining, Derivative Assay